T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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Islets contain four major types of endocrine cells:
Beta Cells - most common and secrete insulin.
Alpha Cells - second most frequent and secrete glucagon.
Delta Cells - secrete somatostatin.
PP Cells - secrete pancreatic polypeptide.
Gomori's stain was an early histochemical technique that distinguished between beta- and alpha-cells. Beta cells stain blue, while alpha cells stain pink. The other types of islet cells cannot be identified.
Today, this technique is primarily of historical interest because of the availability of insulin- and glucagon-specific antibodies for immunohistochemistry.