T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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Ribosomes on its surface indicate this is rough endoplasmic reticulum (RER)
Cytoplasm (green)
False Color
The human eye can distinguish only a limited number of grayscale values but can distinguish between millions of colors. So more information can often be extraced from color images. Thus, pseudo-coloring can be helpful in improving the interpretation of grayscale images.
This image has been colored to highlight the ultrastructure of this cell. The false-colored image is displayed by selecting the “Color” option beneath the image shown above.
Examine the ultrastructure of this spindle-shaped fibroblast:
Because of the wide range of magnifications possible with electron microscopy, it is often difficult to appreciate the size of objects in a micrograph.
Two approaches are usually used to indicate scale:
Magnification Value: the ratio between size of object on a picture and its real size. Because it depends on the final size of the picture, it is only useful with print images.
Scale Bar: a bar of known length is displayed on a picture. Because it is resized when the size of a picture is changed, it is useful with digital images.
A scale bar is shown in the lower left corner. Its length is automatically adjusted as the image is zoomed in or out. In addition, the total size of the view is shown in the right side of the toolbar.
