T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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This is the Golgi apparatus from a pancreatic β cell as seen by transmission electron microscopy (TEM).
Pro-Insulin is synthesized on rough endoplasmic reticulum, transported to the Golgi apparatus by transfer vesicles, moves through the stack of flattened cisterna, and packaged into secretion granules.
Examine the morphology of the following structures:
Transfer Vesicles (purple) - transfer newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus.
Secretion Granules (gray) - the content of immature granules is homogenous, while mature granules have an electron-dense crystal of insulin (black). The mature granules are ~300 µm in diameter. (Note that the morphology of secretion granules varies widely in different cell types.)