MH 012 Nucleic Acids

MICROSCOPE SLIDE

SLIDE NAME

MH 012 Nucleic Acids

TISSUES

Pancreas
Pancreas (DNAse Treated)

STAIN

Fleugen

IMAGE SIZE

27,415 x 41,325
4.2 GB

FILE SIZE

131 MB

MAGNIFICATION

40x

PIXEL SIZE

0.3171 µm

SOURCE

Medical Histology Collection

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Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Version 4.2

© 2005-2019.
T. Clark Brelje and Robert L. Sorenson.
All rights reserved.

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MH 012 Nucleic Acids

Fleugen Reaction

Fleugen stain chemically converts the deoxyribose in DNA into a magenta colored product. This is an example of histochemistry where chemical procedures are used to reveal biochemical characteristics of tissues in situ. This method is both qualitative (identifies the DNA) and quantitative (amount of DNA).

Pancreas

Most of the pancreas is composed of acinar cells. Acinar cells are polarized cells clustered around a central lumen which is the entrance to a duct (i.e., an acinus). Compare the nuclei in acinar cells those within blood vessels and other structures.

Acinar cells have circular nuclei while other cell types have nuclei with elongated shapes.
Red blood cells within the blood vessels do not have nuclei.

Pancreas (DNAse Treated)

This pancreas section was incubated with the enzyme deoxyribose (which hydrolyzes DNA) before Fleugen stainining.

The nuclei of the acinar cells are unstained. This demonstrates that nuclei contain DNA and the Fleugen reaction is relatively specific for staining DNA.