MH 029b Bone

MICROSCOPE SLIDE

SLIDE NAME

MH 029b Bone

TISSUE

Bone

STAIN

Hematoxylin & Eosin

IMAGE SIZE

41,710 x 57,303
8.9 GB

FILE SIZE

353 MB

MAGNIFICATION

40x

PIXEL SIZE

0.3171 µm

SOURCE

Medical Histology Collection

Epiphysis from a long bone.

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Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Version 4.2

© 2005-2019.
T. Clark Brelje and Robert L. Sorenson.
All rights reserved.

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MH 029b Bone

Endochondral Bone Formation

Endochondral bone formation occurs at the epiphyseal plate of long bones. It can be identified by the layer of basophilic hyaline cartilage.

Resting Zone - thin layer of non-dividing chondrocytes.
Proliferative Zone - rapidly dividing chondrocytes (basophilic) that organize into distinct columns ("stacks of coins").
Zone of Hypertrophy - chondrocytes (basophilic) cease dividing and grow in size. The cartilage matrix forms (lightly basophilic) linear bands between the columns of hypertrophied cells.
Zone of Calcification - the cartilage matrix becomes calcified inhibiting the diffusion of nutrients. The dying chondrocytes are removed leaving longitudinal spicules of calcified cartilage (intensely basophilic).
Zone of Ossification - osteoprogenitor cells migrate into the cavities with the new blood vessels. New bone (eosinophilic) forms on the scaffold of calcified cartilage (basophilic).

Osteoblasts - condense on the spicules of calcified cartilage and produce new bone (osteoid).
Osteoclasts (#1, #2 and #3) - large, multinucleated cells that remove bone tissue (both mineralized matrix and type I collagen).