T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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Reticular fibers are composed of type III collagen. They do not stain with H&E, but stain black by metallic silver. Reticular fibers form the stroma of many tissues and organs.
Lymph nodes are an example of tissues with a reticular stroma.
Surrounding the lymph node is a capsule of collagen fibers (type I collagen) stained red.
The outer portion of the lymph node contains aggregations of lymphocytes organized in nodules. Reticular fibers (type III collagen) stained black surround each nodule.
Reticular fibers also support clusters of cells (gray) in the stroma.