T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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The higher contrast staining of the liver with azan helps in the visualization of bile canaliculi. However, other structures tend to be more difficult to visualize.
Lobule - roughly hexagonal structure with a central vein at its center and six portal triads at its periphery.
Central Vein - large venule at the center of the lobule.
Hepatocytes - anastomosing plates, one cell thick, radiate outward from the central vein separated by sinusoidal capillaries and supported by reticular fibers.
Portal Triads - at the corners of each lobule (hepatic arterioles, portal venule, bile ductules and lymphatic vessels).
Bile Ducts - lined with a simple cuboidal epithelium.
Bile Canaliculi (#1 and #2) - bile is secreted into 1 to 2 µm diameter tubes formed by adjacent hepatocytes that drain into bile ductules at the portal triads.
Longitudinal Section - are seen as darker staining lines between adjacent hepatocytes.
Cross-Section - occasionally as a small dot in the center between two hepatocytes.