T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455
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Remodeling of the blood vessel wall in response to vascular injury involves the coordinated regulation of apoptosis, cell recruitment, proliferation, migration, and differentiation.
The response of the femoral artery to mechanical injury with a wire was examined.
The section is from mice with no injury to the femoral artery. It was stained with Masson's trichrome which produces red muscle fibers, blue collagen, light red or pink cytoplasm, and dark brown to black nuclei.
The tunica intima is a thin layer composed of the endothelium (i.e., simple squamous epithelium) and occasional smooth muscle cells inside of the internal elastic membrane.
Courtsey of Neeta Adhikari (Lillehei Heart Institute, University of Minnesota, Minneapolis, MN).
Femoral Artery
The section is from mice with no injury to the femoral artery. It was stained with Verhoef's stain which produces black elastic fibers and light pink/red collagen.
The tunic intima inside of the internal elastic is thinner than the tunica media between the internal and external elastic membranes.
Femoral Artery
This section is from mice 14 days after injury of the femoral artery. It was stained with Masson's trichrome which produces red muscle fibers, blue collagen, light red or pink cytoplasm, and dark brown to black nuclei.
There is thickening of the tunica intima because of proliferation of smooth muscle cells to form an atherosclerotic plaque. This plaque begins to block parts of the vessel lumen.
Femoral Artery
This section is from mice 14 days ater injury of the femoral artery. This section was stained with Verhoef's stain which produces black elastic fibers and light pink/red collagen.
The thicken of the tunica media is more easily seen when the internal and external lamina are stained black.
Femoral Artery
This section is from mice 28 days ater injury of the femoral artery. It was stained with Masson's trichrome which produces red muscle fibers, blue collagen, light red or pink cytoplasm, and dark brown to black nuclei.
The further thickening of the tunic media decreases the vessel lumen. The tunica media is now thicker than the tunica media.
The walls of the artery are so thick and stiff that hypertension results.
