VH 040 Ear

MICROSCOPE SLIDE

SLIDE NAME

VH 040 Ear

TISSUE

Pinna of the Ear
(cow)

STAIN

Masson's Trichrome Stain
Aldehyde Fuchsin

IMAGE SIZE

37,500 x 12,030 pixels
1.3 GB

FILE SIZE

76 MB

MAGNIFICATION

40x

PIXEL SIZE

0.3171 µm

SOURCE

Veterinary Histology Collection

CONTACT

Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Version 4.2

© 2005-2019.
T. Clark Brelje and Robert L. Sorenson.
All rights reserved.

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VH 040 Ear

Elastic Cartilage

Elastic cartilage is similar to hyaline cartilage except that the matrix also contains a dense network of branching and anastomosing elastic fibers. It provides flexible support and is present in the outer ear and epiglottis.

The outer ear (or pinna) stained with Masson's trichrome and aldehyde fuchsin. Masson's trichrome stains connective tissue dark blue, smooth muscle and cytoplasm light pink, and nuclei stain dark red-purple to black. Aldehyde fuchsin stains elastic fibers pink-purple.

Perichondrium (blue) - a layer of dense irregular connective tissue that surrounds cartilage. It is divided into two layers:

Inner Chondrogenic Layer (darker blue) - contains mesenchymal cells that differentiate into chondroblasts, initiate matrix production (elastin and type II collagen) and become immature chondrocytes
Outer Fibrous Layer (blue) - fibroblasts that produce the type I collagen on the outer surface of the perichondrium

Matrix - composed of an extensive, dark pink-purple network of elastic fibers, type II collagen and a ground substance of proteoglycans.
Chondrocytes - cells within lacunae inside the cartilage that occur singularly or in clusters called isogenous groups.

Elastic cartilage contains no blood vessels or nerves.